ARG40709

anti-Nestin antibody

anti-Nestin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,IHC-Frozen sections and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Nestin
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Nestin
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 252-290 of Human Nestin. (ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH)
Conjugation Un-conjugated
Alternate Names Nestin; Nbla00170

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10763 Human NES

GeneID: 18008 Mouse NES

GeneID: 25491 Rat NES

Gene Symbol NES
Gene Full Name nestin
Background This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Calculated MW 177 kDa
PTM Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). [UniProt]