ARG22881
anti-Nestin antibody [Rat-401 (4D4)]
anti-Nestin antibody [Rat-401 (4D4)] for IHC-Frozen sections,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [Rat-401 (4D4)] recognizes Nestin Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. |
|---|---|
| Tested Reactivity | Ms, Rat |
| Species Does Not React With | Hu |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Rat-401 (4D4) |
| Isotype | IgG1 |
| Target Name | Nestin |
| Antigen Species | Rat |
| Immunogen | Nestin purified from embryonic Rat spinal cord. |
| Conjugation | Un-conjugated |
| Alternate Names | Nestin; Nbla00170 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC: We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. WB: Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts. For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified. |
| Buffer | Tris-buffered saline and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Nes |
| Gene Full Name | nestin |
| Background | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). |
