ARG53358

anti-Nestin antibody [SP103]

anti-Nestin antibody [SP103] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
publication_link Publication1

Overview

Product Description Rabbit Monoclonal antibody [SP103] recognizes Nestin
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP103
Isotype IgG
Target Name Nestin
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of human nestin.
Conjugation Un-conjugated
Alternate Names Nestin; Nbla00170

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
WB1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Kidney, HeLa Cell Lysate

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10763 Human NES

Swiss-port # P48681 Human Nestin

Gene Symbol NES
Gene Full Name nestin
Background This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). [UniProt]
Cellular Localization Cytoplasm
Highlight Related Antibody Duos and Panels:
ARG30083 Neuron Development Marker Antibody Duo (Nestin, Tau)
Related products:
Nestin antibodies; Nestin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Calculated MW 177 kDa
PTM Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

Specific References

In Vivo Melanoma Cell Morphology Reflects Molecular Signature and Tumor Aggressiveness

IHC-P / Human

Alessandra Marconi et al.
J Invest Dermatol.,  (2022)

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