ARG53358
anti-Nestin antibody [SP103]
anti-Nestin antibody [SP103] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Publication1
Overview
| Product Description | Rabbit Monoclonal antibody [SP103] recognizes Nestin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP103 |
| Isotype | IgG |
| Target Name | Nestin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminus of human nestin. |
| Conjugation | Un-conjugated |
| Alternate Names | Nestin; Nbla00170 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Kidney, HeLa Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NES |
| Gene Full Name | nestin |
| Background | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm |
| Highlight | Related Antibody Duos and Panels: ARG30083 Neuron Development Marker Antibody Duo (Nestin, Tau) Related products: Nestin antibodies; Nestin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Neuronal Development Marker Fight microcephaly with arigo |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). |
Specific References
In Vivo Melanoma Cell Morphology Reflects Molecular Signature and Tumor Aggressiveness
IHC-P / Human
