ARG56065
anti-Neurofilament NF-H (Phosphorylated) antibody [NE14]
anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Overview
| Product Description | Mouse Monoclonal antibody [NE14] recognizes Neurofilament NF-H (Phosphorylated) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NE14 |
| Isotype | IgG1 |
| Target Name | Neurofilament NF-H |
| Antigen Species | Pig |
| Immunogen | Crude neurofilament preparation from Pig spinal cord. |
| Conjugation | Un-conjugated |
| Alternate Names | Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human brain |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | NEFH |
| Gene Full Name | neurofilament, heavy polypeptide |
| Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Research Area | Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| Calculated MW | 112 kDa |
| PTM | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
