ARG55971

anti-Neurofilament NF-H antibody [RT97]

anti-Neurofilament NF-H antibody [RT97] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody

Overview

Product Description

Mouse Monoclonal antibody [RT97] recognizes Neurofilament NF-H

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone RT97
Isotype IgG1
Target Name Neurofilament NF-H
Antigen Species Rat
Immunogen The triton-X 100 insoluble protein fraction of Rat brain.
Conjugation Un-conjugated
Alternate Names Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human brain

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 380684 Mouse NEFH

GeneID: 4744 Human NEFH

Swiss-port # P12036 Human Neurofilament heavy polypeptide

Swiss-port # P19246 Mouse Neurofilament heavy polypeptide

Gene Symbol Nefh
Gene Full Name neurofilament, heavy polypeptide
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt]
Cellular Localization Cytoplasmic
Highlight Related products:
Neurofilament NF H antibodies; Neurofilament NF H ELISA Kits; Neurofilament NF H Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Neuronal Development Marker
Research Area Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Calculated MW 112 kDa
PTM There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.