ARG11132

anti-Neurofilament NF-H phospho (KSP site) antibody [9B12]

anti-Neurofilament NF-H phospho (KSP site) antibody [9B12] for Western blot,ICC/IF,IHC-Frozen sections and Human,Rat,Mouse,Cow

Overview

Product Description Mouse Monoclonal antibody [9B12] recognizes Neurofilament NF-H phospho (KSP site)
Tested Reactivity Hu, Ms, Rat, Cow
Tested Application ICC/IF, IHC-Fr, WB
Specificity This antibody recognizes the phosphorylated NF-H KSP sequences. In some species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M.
Host Mouse
Clonality Monoclonal
Clone 9B12
Isotype IgG2b
Target Name Neurofilament NF-H
Antigen Species Bovine
Immunogen Native Neurofilament NF-H purified from bovine spinal cord, binding to phosphorylated KSP sequences.
Conjugation Un-conjugated
Alternate Names Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 380684 Mouse NEFH

GeneID: 4744 Human NEFH

Swiss-port # P12036 Human Neurofilament heavy polypeptide

Swiss-port # P19246 Mouse Neurofilament heavy polypeptide

Gene Symbol NEFH
Gene Full Name neurofilament, heavy polypeptide
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 112 kDa
PTM There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. [UniProt]