ARG52346

anti-Neurofilament NF-H phospho (KSP site) antibody [NAP4]

anti-Neurofilament NF-H phospho (KSP site) antibody [NAP4] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Cow,Mouse,Pig,Rat

Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody

Overview

Product Description

Mouse Monoclonal antibody [NAP4] recognizes Neurofilament NF-H when KSP site was phosphorylated

Tested Reactivity Ms, Rat, Cow, Pig
Predict Reactivity Hu
Tested Application IHC-P, WB
Specificity NAP4 recognizes phosphorylated NF-H KSP sequences but not non-phosphorylated KSP sequences.
Host Mouse
Clonality Monoclonal
Clone NAP4
Isotype IgG1
Target Name Neurofilament NF-H
Antigen Species Bovine
Immunogen Purified bovine NF-H
Conjugation Un-conjugated
Alternate Names Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:500 - 1:1000
WB1:5000 - 1:10000
Application Note Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Total IgG fraction
Buffer Total IgG fraction and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100156492 Pig NEFH

GeneID: 100625407 Pig NEFH

GeneID: 380684 Mouse NEFH

Swiss-port # P19246 Mouse Neurofilament heavy polypeptide

Background Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease .
Research Area Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Calculated MW 112 kDa
PTM There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Clone References

Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.

WB / Rat

Benson DL et al.
J Neurocytol.,  (1996)

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