ARG11140
anti-Neurofilament NF-L (C-ter) antibody
anti-Neurofilament NF-L (C-ter) antibody for Western blot,IHC-Frozen sections and Human,Rat,Mouse,Cow,Pig
Overview
Product Description | Rabbit Polyclonal antibody recognizes Neurofilament NF-L (C-ter) |
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Tested Reactivity | Hu, Ms, Rat, Cow, Pig |
Tested Application | IHC-Fr, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Neurofilament NF-L (C-ter) |
Antigen Species | Rat |
Immunogen | KLH-conjugated synthetic peptide around the C-terminal region of Rat Neurofilament NF-L. (C-GEEEDTKESEEEEKKEESAGEEQAAKKKD) |
Conjugation | Un-conjugated |
Alternate Names | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Observed Size | ~ 63 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
Preservative | 5 mM Sodium azide |
Stabilizer | 50% Glycerol |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | NEFL |
Gene Full Name | neurofilament, light polypeptide |
Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt] |
Calculated MW | 62 kDa |
PTM | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. [UniProt] |