ARG11122

anti-Neurofilament NF-L antibody [1B11]

anti-Neurofilament NF-L antibody [1B11] for Western blot,ICC/IF,IHC-Frozen sections and Human,Rat,Mouse,Cow,Pig,Horse

Overview

Product Description Mouse Monoclonal antibody [1B11] recognizes Neurofilament NF-L
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 1B11
Isotype IgG1
Target Name Neurofilament NF-L
Antigen Species Pig
Immunogen Pig Neurofilament NF-L.
Epitope Within the C terminal segment of alpha-helical region, a highly conserved region known as Coil II, corresponding to aa. 256-400 of the human protein.
Conjugation Un-conjugated
Alternate Names Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000
IHC-Fr1:2000
WB1:10000 - 1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 62 kDa

Properties

Form Liquid
Purification Purified
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100521224 Pig NEFL

GeneID: 18039 Mouse NEFL

GeneID: 4747 Human NEFL

Gene Symbol NEFL
Gene Full Name neurofilament, light polypeptide
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt]
Calculated MW 62 kDa
PTM O-glycosylated.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Ubiquitinated in the presence of TRIM2 and UBE2D1. [UniProt]