ARG52349

anti-Neurofilament NF-L antibody

anti-Neurofilament NF-L antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody

Overview

Product Description Chicken Polyclonal antibody recognizes Neurofilament NF-L
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Neurofilament NF-L
Antigen Species Bovine
Immunogen Preparation of bovine spinal cord NF-L
Conjugation Un-conjugated
Alternate Names Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:2000 - 1:5000
IHC-Fr1:2000
WB1:5000 - 1:10000
Application Note Specific for the ~ 68 kDa Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 68 kDa

Properties

Form Liquid
Purification Total IgY fraction
Buffer Total IgY fraction in PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18039 Mouse NEFL

GeneID: 4747 Human NEFL

GeneID: 83613 Rat NEFL

Gene Symbol NEFL
Gene Full Name neurofilament, light polypeptide
Background Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease .
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt]
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Calculated MW 62 kDa
PTM O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.