ARG52348
anti-Neurofilament NF-L antibody [DA2]
anti-Neurofilament NF-L antibody [DA2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Overview
| Product Description | Mouse Monoclonal antibody [DA2] recognizes Neurofilament NF-L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DA2 |
| Isotype | IgG1 |
| Target Name | Neurofilament NF-L |
| Antigen Species | Pig |
| Immunogen | Preparation of porcine NF-L |
| Epitope | Epitope is likely centered on the HVQEEQIEVE peptide. |
| Conjugation | Un-conjugated |
| Alternate Names | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~68k Neurofilament L protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Total IgG fraction |
| Buffer | Total IgG fraction and 10 mM Sodium azide |
| Preservative | 10 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease . |
| Highlight | Related Antibody Duos and Panels: ARG30010 Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII) ARG30011 Neurofilament Antibody Duo (NF-H, NF-L) ARG30145 Intermediate Neurofilament Antibody Panel (NF-L, NF-M,NF-H) Related products: Neurofilament antibodies; Neurofilament ELISA Kits; Neurofilament Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| Calculated MW | 62 kDa |
| PTM | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
Customer's Feedback
Excellent
Review for anti-Neurofilament NF-L antibody [DA2]
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
WB / Human
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
WB / Rat
Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system.
ICC/IF / Chicken
