ARG52351

anti-Neurofilament NF-M antibody

anti-Neurofilament NF-M antibody for ICC/IF,IHC-Frozen sections,Western blot and Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Intermediate Neurofilament antibody

Overview

Product Description Chicken Polyclonal antibody recognizes Neurofilament NF-M
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Chk, Cow, Hrs, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Neurofilament NF-M
Antigen Species Rat
Immunogen Preparation containing the extreme C-terminus expressed in and purified from E. Coli
Conjugation Un-conjugated
Alternate Names Neurofilament medium polypeptide; Neurofilament 3; Neurofilament triplet M protein; NFM; NF-M; 160 kDa neurofilament protein; NEF3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:2000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat cortex
Observed Size ~ 145 kDa

Properties

Form Liquid
Purification Total IgY fraction
Buffer Total IgY fraction in PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18040 Mouse NEFM

GeneID: 24588 Rat NEFM

Swiss-port # P08553 Mouse Neurofilament medium polypeptide

Swiss-port # P12839 Rat Neurofilament medium polypeptide

Gene Symbol Nefm
Gene Full Name neurofilament, medium polypeptide
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30145 Intermediate Neurofilament Antibody Panel (NF-L, NF-M,NF-H)
Related products:
Neurofilament antibodies; Neurofilament ELISA Kits; Neurofilament Duos / Panels; Anti-Chicken IgY secondary antibodies;
Related news:
Neuronal Development Marker
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Intermediate Neurofilament antibody
Calculated MW 102 kDa
PTM There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.