ARG66559

anti-Neurotensin Receptor 1 antibody

anti-Neurotensin Receptor 1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Neurotensin Receptor 1
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Neurotensin Receptor 1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of Human NTR1.
Conjugation Un-conjugated
Alternate Names NTR; NTR1; Neurotensin receptor type 1; NT-R-1; NTRH; High-affinity levocabastine-insensitive neurotensin receptor

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:500
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 54 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4923 Human NTSR1

Swiss-port # P30989 Human Neurotensin receptor type 1

Gene Symbol NTSR1
Gene Full Name neurotensin receptor 1 (high affinity)
Background Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. [provided by RefSeq, Jul 2008]
Function G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. Membrane raft. Note=Palmitoylation is required for localization at CAV1-enriched membrane rafts. [UniProt]
Calculated MW 46 kDa
PTM N-glycosylated.

Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment. [UniProt]