ARG62566

anti-Neutrophil Elastase antibody [NP57]

anti-Neutrophil Elastase antibody [NP57] for Western blot,Immunohistochemistry and Human

Microbiology and Infectious Disease antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [NP57] recognizes Neutrophil Elastase
Tested Reactivity Hu
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone NP57
Isotype IgG1, kappa
Target Name Neutrophil Elastase
Antigen Species Human
Immunogen Human neutrophil granule proteins
Conjugation Un-conjugated
Alternate Names Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1991 Human ELANE

Swiss-port # P08246 Human Neutrophil elastase

Gene Symbol ELANE
Gene Full Name elastase, neutrophil expressed
Background Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. [UniProt]
Research Area Microbiology and Infectious Disease antibody; Signaling Transduction antibody
Calculated MW 29 kDa