ARG54245
anti-Nitrotyrosine antibody [EM-30]
anti-Nitrotyrosine antibody [EM-30] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Broad
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [EM-30] recognizes Nitrotyrosine |
|---|---|
| Tested Reactivity | Broad |
| Tested Application | IHC-Fr, IHC-P, WB |
| Specificity | The clone EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-30 |
| Isotype | IgG1 |
| Target Name | Nitrotyrosine |
| Immunogen | NO2-Tyr-CH2-Thyroglobulin |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | WB: NO2-BSA and NO2-HSA IHC-P and IHC-Fr: Colon cancer epithelium, prostate hyperplasia |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage. |
|---|---|
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
