ARG56172
anti-Nkx 2.2 antibody [NX2/294]
anti-Nkx 2.2 antibody [NX2/294] for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody [NX2/294] recognizes Nkx 2.2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NX2/294 |
| Isotype | IgG2b, kappa |
| Target Name | Nkx 2.2 |
| Antigen Species | Human |
| Immunogen | Human Nkx2.2 recombinant protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Homeobox protein NK-2 homolog B; NKX2.2; Homeobox protein Nkx-2.2; NKX2B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NKX2-2 |
| Gene Full Name | NK2 homeobox 2 |
| Background | The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008] |
| Function | Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt] |
| Cellular Localization | Nuclear |
| Calculated MW | 30 kDa |
