ARG63263

anti-Npap60 / Nup50 antibody

anti-Npap60 / Nup50 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Npap60 / Nup50
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Npap60 / Nup50
Antigen Species Human
Immunogen C-ELHKILLEKKDA
Conjugation Un-conjugated
Alternate Names Nuclear pore complex protein Nup50; 50 kDa nucleoporin; NPAP60L; Nucleoporin Nup50; NPAP60; Nuclear pore-associated protein 60 kDa-like

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
WB0.2 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10762 Human NUP50

Swiss-port # Q9UKX7 Human Nuclear pore complex protein Nup50

Background The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 50 kDa