ARG52376
anti-Nuclei antibody [235-1]
anti-Nuclei antibody [235-1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Controls and Markers antibody
Overview
| Product Description | Mouse Monoclonal antibody [235-1] recognizes Nuclei |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 235-1 |
| Isotype | IgG1 |
| Target Name | Nuclei |
| Antigen Species | Human |
| Immunogen | Human cell homogenate. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Total IgG fraction |
| Buffer | Total IgG fraction |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | marker for human cells in xenographic research |
|---|---|
| Background | This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. |
| Research Area | Controls and Markers antibody |
