ARG40529

anti-OGDH antibody

anti-OGDH antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

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Overview

Product Description Rabbit Polyclonal antibody recognizes OGDH
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name OGDH
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 148-427 of Human OGDH (NP_001003941.1).
Conjugation Un-conjugated
Alternate Names 2-oxoglutarate dehydrogenase complex component E1; EC 1.2.4.2; AKGDH; OGDC-E1; OGDC; 2-oxoglutarate dehydrogenase, mitochondrial; E1k; Alpha-ketoglutarate dehydrogenase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain
Observed Size 110 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18293 Mouse OGDH

GeneID: 360975 Rat OGDH

GeneID: 4967 Human OGDH

Gene Symbol OGDH
Gene Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Background This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Function The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). [UniProt]
Cellular Localization Mitochondrion matrix. Nucleus. Note=Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation. [UniProt]
Calculated MW 116 kDa

Specific References

The effect of ochratoxin A on cytotoxicity and glucose metabolism in human esophageal epithelium Het-1A cells

WB / Human

Man Zhao et al.
Toxicon.,  (2021)

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Ochratoxin A induces reprogramming of glucose metabolism by switching energy metabolism from oxidative phosphorylation to glycolysis in human gastric epithelium GES-1 cells in vitro.

WB / Human

Yuan Wang et al.
Toxicol Lett.,  (2020)

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