ARG64143

anti-OGT antibody

anti-OGT antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes OGT
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Cow, Dog
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name OGT
Antigen Species Human
Immunogen C-YEHPKDLKLSDGR
Conjugation Un-conjugated
Alternate Names UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GLCNAC; EC 2.4.1.255; OGT; HRNT1; HINCUT-1; O-linked N-acetylglucosamine transferase 110 kDa subunit; O-GlcNAc transferase subunit p110

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P5 µg/ml
WB0.05 - 0.2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26295 Rat OGT

GeneID: 8473 Human OGT

Swiss-port # O15294 Human UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa s

Swiss-port # P56558 Rat UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa sub

Background This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 117 kDa
PTM Ubiquitinated, leading to its proteasomal degradation.
Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.