ARG66155

anti-OPN / Osteopontin antibody (Biotin)

anti-OPN / Osteopontin antibody (Biotin) for ELISA and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes OPN / Osteopontin
Tested Reactivity Hu
Tested Application ELISA
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name OPN / Osteopontin
Antigen Species Human
Immunogen HEK293 cells derived recombinant Human Osteopontin.
(IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN)
Conjugation Biotin
Alternate Names BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: ~ 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66154 as a capture antibody
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6696 Human SPP1

Swiss-port # P10451 Human Osteopontin

Gene Symbol SPP1
Gene Full Name secreted phosphoprotein 1
Background The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt]
Cellular Localization Secreted. [UniProt]
Highlight Related products:
OPN antibodies; OPN ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
The role of HDGF in tumor angiogenesis
Calculated MW 35 kDa
PTM Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.
N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.