ARG62573

anti-ORC1 antibody [7F6/1]

anti-ORC1 antibody [7F6/1] for Western blot and Human

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [7F6/1] recognizes ORC1
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 7F6/1
Isotype IgG1
Target Name ORC1
Antigen Species Human
Immunogen Full length protein (Human)
Conjugation Un-conjugated
Alternate Names ORC1L; Replication control protein 1; PARC1; Origin recognition complex subunit 1; HSORC1

Application Instructions

Application Note WB: 1/1250 - 1/2500
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification IgG purified
Buffer PBS
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4998 Human ORC1

Swiss-port # Q13415 Human Origin recognition complex subunit 1

Gene Symbol ORC1
Gene Full Name origin recognition complex, subunit 1
Background The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Function Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. [UniProt]
Cellular Localization Nucleus
Research Area Gene Regulation antibody
Calculated MW 97 kDa
PTM Phosphorylated during mitosis.

Clone References

The role of PR-Set7 in replication licensing depends on Suv4-20h.

WB, ChIP / Human

Beck DB et al.
Genes Dev.,  (2012)

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HOXD13 binds DNA replication origins to promote origin licensing and is inhibited by geminin.

ChIP / Human, Mouse

Salsi V et al.
Mol Cell Biol.,  (2009)

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