ARG40512

anti-Ogg1 antibody

anti-Ogg1 antibody for Western blot and Human,Mouse

publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes Ogg1
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ogg1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide between aa. 318-345 of Human Ogg1.
Conjugation Un-conjugated
Alternate Names EC 4.2.99.18; N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase; HOGG1; MUTM; OGH1; AP lyase; HMMH; apurinic or apyrimidinic site; EC 3.2.2.-

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18294 Mouse OGG1

GeneID: 4968 Human OGG1

Swiss-port # O08760 Mouse N-glycosylase/DNA lyase

Swiss-port # O15527 Human N-glycosylase/DNA lyase

Gene Symbol OGG1
Gene Full Name 8-oxoguanine DNA glycosylase
Background This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
Function DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. [UniProt]
Cellular Localization Nucleus, nucleoplasm. Nucleus speckle. Nucleus matrix. Note=Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. Isoform 1A: Nucleus. Isoform 2A: Mitochondrion. [UniProt]
Calculated MW 39 kDa

Specific References

Propolis alleviates 4-aminobiphenyl-induced oxidative DNA damage by inhibition of CYP2E1 expression in human liver cells

WB / Human

Eva Ari Wahyuni et al.
Environ Toxicol.,  (2021)

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