ARG64260

anti-Ogg1 antibody

anti-Ogg1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Cancer antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes Ogg1
Tested Reactivity Hu, Rat
Predict Reactivity Ms
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Ogg1
Antigen Species Mouse
Immunogen C-HPKTSQAKGPSPLANK
Conjugation Un-conjugated
Alternate Names EC 4.2.99.18; N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase; HOGG1; MUTM; OGH1; AP lyase; HMMH; apurinic or apyrimidinic site; EC 3.2.2.-

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4968 Human OGG1

GeneID: 81528 Rat OGG1

Swiss-port # O15527 Human N-glycosylase/DNA lyase

Swiss-port # O70249 Rat N-glycosylase/DNA lyase

Gene Symbol Ogg1
Gene Full Name 8-oxoguanine DNA-glycosylase 1
Background This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
Function DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. [UniProt]
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 39 kDa