ARG22576
anti-P2RX7 / P2X7 Receptor antibody [Hano43]
anti-P2RX7 / P2X7 Receptor antibody [Hano43] for Flow cytometry,ICC/IF and Mouse
Overview
| Product Description | Rat Monoclonal antibody [Hano43] recognizes P2RX7 / P2X7 Receptor |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, ICC/IF |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | Hano43 |
| Isotype | IgG2b |
| Target Name | P2RX7 / P2X7 Receptor |
| Antigen Species | Mouse |
| Immunogen | A P2X7-expression construct and a final boost with P2X7-transfected HEK cells. |
| Conjugation | Un-conjugated |
| Alternate Names | ATP receptor; P2X7; P2X purinoceptor 7; P2Z receptor; Purinergic receptor |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | P2rx7 |
| Gene Full Name | purinergic receptor P2X, ligand-gated ion channel, 7 |
| Background | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010] |
| Function | Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. |
