ARG59851
anti-P4HA3 antibody
anti-P4HA3 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes P4HA3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | P4HA3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 20-110 of Human P4HA3 (NP_878907.1). |
| Conjugation | Un-conjugated |
| Alternate Names | 4-PH alpha-3; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Prolyl 4-hydroxylase subunit alpha-3; EC 1.14.11.2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | 293T | ||||||
| Observed Size | 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6W3E9 Rat Prolyl 4-hydroxylase subunit alpha-3 Swiss-port # Q7Z4N8 Human Prolyl 4-hydroxylase subunit alpha-3 |
|---|---|
| Gene Symbol | P4HA3 |
| Gene Full Name | prolyl 4-hydroxylase, alpha polypeptide III |
| Background | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. [UniProt] |
| Cellular Localization | Endoplasmic reticulum lumen. [UniProt] |
| Calculated MW | 61 kDa |
| PTM | N-glycosylation plays no role in the catalytic activity. [UniProt] |
