ARG63084
anti-PAG / Cbp antibody [MEM-255]
anti-PAG / Cbp antibody [MEM-255] for Flow cytometry,Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-255] recognizes PAG / Cbp |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat, Bov |
| Tested Application | FACS, IHC-P, WB |
| Specificity | The clone MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-255 |
| Isotype | IgG2a |
| Target Name | PAG / Cbp |
| Antigen Species | Human |
| Immunogen | Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG). |
| Conjugation | Un-conjugated |
| Alternate Names | Transmembrane phosphoprotein Cbp; Csk-binding protein; CBP; PAG; Transmembrane adapter protein PAG; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Intracellular staining, permeabilization required. WB: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Tonsil and spleen |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NWQ8 Human Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 |
|---|---|
| Gene Symbol | PAG1 |
| Gene Full Name | phosphoprotein membrane anchor with glycosphingolipid microdomains 1 |
| Background | PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. |
| Function | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 47 kDa |
| PTM | Palmitoylated. Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN. |
Clone References
Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor.
Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton.
Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.
