ARG63744
anti-PAI1 / SERPINE1 antibody
anti-PAI1 / SERPINE1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PAI1 / SERPINE1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAI1 / SERPINE1 |
| Antigen Species | Human |
| Immunogen | C-GFKIDDKGMAPALRH |
| Conjugation | Un-conjugated |
| Alternate Names | Serpin E1; PLANH1; Endothelial plasminogen activator inhibitor; Plasminogen activator inhibitor 1; PAI; PAI1; PAI-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody |
| Calculated MW | 45 kDa |
| PTM | Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond. |
