ARG66125

anti-PAI1 / SERPINE1 antibody (Biotin)

anti-PAI1 / SERPINE1 antibody (Biotin) for ELISA and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes PAI1 / SERPINE1
Tested Reactivity Hu
Tested Application ELISA
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PAI1 / SERPINE1
Antigen Species Human
Immunogen E. coli derived recombinant Human PAI1 / SERPINE1.
(VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEP)
Conjugation Biotin
Alternate Names Serpin E1; PLANH1; Endothelial plasminogen activator inhibitor; Plasminogen activator inhibitor 1; PAI; PAI1; PAI-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66124 as a capture antibody
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5054 Human SERPINE1

Swiss-port # P05121 Human Plasminogen activator inhibitor 1

Gene Symbol SERPINE1
Gene Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Background This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Function Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. [UniProt]
Calculated MW 45 kDa
PTM Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.