ARG52387

anti-PAK1 / PAK2 / PAK3 phospho (Thr402) antibody

anti-PAK1 / PAK2 / PAK3 phospho (Thr402) antibody for Western blot and Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PAK1 / PAK2 / PAK3 phospho (Thr402)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Dog
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PAK1 / PAK2 / PAK3
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH
Conjugation Un-conjugated
Alternate Names PAKalpha; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p65-PAK; EC 2.7.11.1; PAK-1; p21-activated kinase 1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~68k to ~70k PAK protein phosphorylated at Thr402. The immunolabeling of PAK is completely eliminated by λ-phosphatase treatment.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29431 Rat PAK1

Swiss-port # P35465 Rat Serine/threonine-protein kinase PAK 1

Gene Symbol PAK1-3
Gene Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Background In mammals, there are several identified isoforms of p21-activated protein k inases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brainspecific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 61 kDa
PTM Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites.