ARG51663
anti-PAK1 / PAK2 / PAK3 phospho (Thr423 / Thr402 / Thr421) antibody
anti-PAK1 / PAK2 / PAK3 phospho (Thr423 / Thr402 / Thr421) antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PAK1 / PAK2 / PAK3 phospho (Thr423 / Thr402 / Thr421) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAK1 / PAK2 / PAK3 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T(p)-M-V) derived from Human PAK1/PAK2/PAK3. |
| Conjugation | Un-conjugated |
| Alternate Names | beta-PAK; bPAK; MRX30; PAK3beta; OPHN3; Oligophrenin-3; MRX47; Serine/threonine-protein kinase PAK 3; EC 2.7.11.1; PAK-3; p21-activated kinase 3; Beta-PAK |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
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| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | PAK3 |
| Gene Full Name | p21 protein (Cdc42/Rac)-activated kinase 3 |
| Background | The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. |
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 62 kDa |
| PTM | Autophosphorylated when activated by CDC42/p21. Neddylated. |
