ARG52386

anti-PAK1 phospho (Thr84) antibody

anti-PAK1 phospho (Thr84) antibody for Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PAK1 phospho (Thr84)
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Rat, Chk, Dog, NHuPrm, Sheep, Zfsh
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PAK1
Antigen Species Human
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 conjugated to KLH
Conjugation Un-conjugated
Alternate Names PAKalpha; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p65-PAK; EC 2.7.11.1; PAK-1; p21-activated kinase 1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~68k PAK1 phosphorylated at Thr84.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5058 Human PAK1

Swiss-port # Q13153 Human Serine/threonine-protein kinase PAK 1

Gene Symbol PAK1
Gene Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Background PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin (Chen et al, 2004).
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 61 kDa
PTM Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites.