ARG42665

anti-PAK3 antibody

anti-PAK3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PAK3
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Specificity ARG42665 anti-PAK3 antibody reacts to PAK1 with low binding affinity and not react to PAK2.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PAK3
Antigen Species Human
Immunogen Synthetic peptide within aa. 1-50 of Human PAK3.
Conjugation Un-conjugated
Alternate Names beta-PAK; bPAK; MRX30; PAK3beta; OPHN3; Oligophrenin-3; MRX47; Serine/threonine-protein kinase PAK 3; EC 2.7.11.1; PAK-3; p21-activated kinase 3; Beta-PAK

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human fetal brain
Observed Size ~ 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18481 Mouse PAK3

GeneID: 29433 Rat PAK3

GeneID: 5063 Human PAK3

Gene Symbol PAK3
Gene Full Name p21 protein (Cdc42/Rac)-activated kinase 3
Background The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017]
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 62 kDa
PTM Autophosphorylated when activated by CDC42/p21.
Neddylated. [UniProt]