ARG57124

anti-PARP2 antibody [29G4]

anti-PARP2 antibody [29G4] for Western blot,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [29G4] recognizes PARP2
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 29G4
Isotype IgG2a, kappa
Target Name PARP2
Antigen Species Human
Immunogen Recombinant fragment around aa. 233-583 of Human PARP2
Conjugation Un-conjugated
Alternate Names EC 2.4.2.30; hPARP-2; ARTD2; NAD; pADPRT-2; PARP-2; Poly [ADP-ribose] polymerase 2; Poly[ADP-ribose] synthase 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT-2; ADPRTL2; ADPRTL3; ADPRT2

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WB1:250 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10038 Human PARP2

Swiss-port # Q9UGN5 Human Poly [ADP-ribose] polymerase 2

Gene Symbol PARP2
Gene Full Name poly (ADP-ribose) polymerase 2
Background This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]
Function Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. [UniProt]
Calculated MW 66 kDa
PTM Poly-ADP-ribosylated by PARP1.
Acetylation reduces DNA binding and enzymatic activity.