ARG56518
anti-PCK1 / PEPCKC antibody
anti-PCK1 / PEPCKC antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PCK1 / PEPCKC |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PCK1 / PEPCKC |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide around the N-terminus of Mouse PCK1 / PEPCK-C. |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; EC 4.1.1.32; PEPCK1; PEPCK-C; PEPCKC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | TBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.1% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07379 Rat Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Swiss-port # Q9Z2V4 Mouse Phosphoenolpyruvate carboxykinase, cytosolic [GTP] |
|---|---|
| Gene Symbol | Pck1 |
| Gene Full Name | phosphoenolpyruvate carboxykinase 1, cytosolic |
| Background | This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2. Ubiquitination by UBR5 leads to proteasomal degradation. |
