ARG42375
anti-PDE8A antibody [EM-52]
anti-PDE8A antibody [EM-52] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [EM-52] recognizes PDE8A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | The mouse monoclonal antibody EM-52 recognizes PDE8a (phosphodiesterase 8 A), an approximately 93 kDa intracellular enzyme that hydrolyzes cAMP to 5'AMP. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-52 |
| Isotype | IgG1, kappa |
| Target Name | PDE8A |
| Antigen Species | Human |
| Immunogen | Human PDE8A. |
| Conjugation | Un-conjugated |
| Alternate Names | HsT19550; High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; EC 3.1.4.53 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60658 Human High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiest |
|---|---|
| Gene Symbol | PDE8A |
| Gene Full Name | phosphodiesterase 8A |
| Background | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:18983167). May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed:18983167). Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed:23509299). Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed:23509299). [UniProt] |
| Calculated MW | 93 kDa |
| PTM | Phosphorylated at Ser-359 by PKA under elevated cAMP conditions, this enhances catalytic activity. [UniProt] |
