ARG66011

anti-PDGF AA antibody

anti-PDGF AA antibody for ELISA,Neutralizing,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PDGF AA
Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDGF AA
Antigen Species Human
Immunogen E. coli derived recombinant Human PDGF AA.
(SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT)
Conjugation Un-conjugated
Alternate Names Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; PDGF-1; PDGF1; PDGF-A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66012 as a detection antibody
IHC-P1 µg/ml
Neut0.13 - 0.20 µg/ml (To yield [ND50] of the biological activity of hPDGF-AA (5.0 ng/ml) )
Application Note IHC-P: Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5154 Human PDGFA

Swiss-port # P04085 Human Platelet-derived growth factor subunit A

Gene Symbol PDGFA
Gene Full Name platelet-derived growth factor alpha polypeptide
Background The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity). [UniProt]
Calculated MW 24 kDa