ARG63746

anti-PDHX / Pyruvate dehydrogenase antibody

anti-PDHX / Pyruvate dehydrogenase antibody for Western blot and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes PDHX / Pyruvate dehydrogenase
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application WB
Specificity This antibody is expected to recognise all reported isoforms.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PDHX / Pyruvate dehydrogenase
Antigen Species Human
Immunogen C-KSFKANLENPIRLA
Conjugation Un-conjugated
Alternate Names Lipoyl-containing pyruvate dehydrogenase complex component X; OPDX; proX; E3-binding protein; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3BP; PDX1; Pyruvate dehydrogenase protein X component, mitochondrial; DLDBP

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8050 Human PDHX

Swiss-port # O00330 Human Pyruvate dehydrogenase protein X component, mitochondrial

Background The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 54 kDa
PTM Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.