ARG65748

anti-PDI antibody

anti-PDI antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

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Overview

Product Description

Rabbit Polyclonal antibody recognizes PDI

Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDI
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to a region of Human PDI (NP_006840.2).
Conjugation Un-conjugated
Alternate Names PDA2; Pancreas-specific protein disulfide isomerase; PDIR; PDIP; PDI; Protein disulfide-isomerase A2; PDIp; EC 5.3.4.1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200
IHC-P1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, MCF-7, PANC, HepG2, HUVEC, NIH/3T3, L929, F9, Jurkat, A431, A549, Mouse liver tissue, Mouse kidney tissue, Mouse brain tissue, Human lung carcinoma tissue, Human kidney, Human brain, Human liver, Human colon carcinoma tissue

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer TBS (pH 7.4), 0.05% Sodium azide, 40% Glycerol and 1% BSA.
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol and 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64714 Human PDIA2

GeneID: 69191 Mouse PDIA2

Swiss-port # D3Z6P0 Mouse Protein disulfide-isomerase A2

Swiss-port # Q13087 Human Protein disulfide-isomerase A2

Gene Symbol PDIA2
Gene Full Name protein disulfide isomerase family A, member 2
Background Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]). [supplied by OMIM, Mar 2008]
Function Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. [UniProt]
Calculated MW 58 kDa
PTM The disulfide-linked homodimer exhibits an enhanced chaperone activity.
Glycosylated.

Customer's Feedback

nuts_pic      Excellent

Review for anti-PDI antibody

Application:WB

Sample:Mouse liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-PDI antibody

Application:WB

Sample:MCF-7

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:3000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC