ARG42504

anti-PDIA3 / ERp57 antibody

anti-PDIA3 / ERp57 antibody for Western blot,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human,Mouse,Rat,Dog,Monkey

Overview

Product Description Goat Polyclonal antibody recognizes PDIA3 / ERp57
Tested Reactivity Hu, Ms, Rat, Dog, Mk
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PDIA3 / ERp57
Antigen Species Human
Immunogen Recombinante peptide within aa. 300 to the C-terminus of Human PDIA3 / ERp57.
Conjugation Un-conjugated
Alternate Names EC 5.3.4.1; Disulfide isomerase ER-60; HEL-S-93n; GRP57; p58; Endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; ERp57; GRP58; P58; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER60; HEL-S-269; Protein disulfide-isomerase A3; PI-PLC; ERp60; ERp61; HsT17083; Endoplasmic reticulum resident protein 57

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:500
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Hepa and TR-iBRB
Observed Size ~ 57 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14827 Mouse PDIA3

GeneID: 2923 Human PDIA3

GeneID: 29468 Rat PDIA3

Gene Symbol PDIA3
Gene Full Name protein disulfide isomerase family A, member 3
Background This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
Cellular Localization Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). [UniProt]
Calculated MW 57 kDa