ARG42546

anti-PDIA6 antibody

anti-PDIA6 antibody for Western blot and Human,Mouse,Rat,Dog,Monkey

Overview

Product Description Goat Polyclonal antibody recognizes PDIA6
Tested Reactivity Hu, Ms, Rat, Dog, Mk
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PDIA6
Antigen Species Human
Immunogen Recombinant peptide within aa. 220 to the C-terminus of Human PDIA6.
Conjugation Un-conjugated
Alternate Names Protein disulfide isomerase P5; ERP5; Thioredoxin domain-containing protein 7; P5; ER protein 5; Endoplasmic reticulum protein 5; ERp5; EC 5.3.4.1; Protein disulfide-isomerase A6; TXNDC7

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and HT1080
Observed Size ~ 49 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10130 Human PDIA6

GeneID: 286906 Rat PDIA6

GeneID: 71853 Mouse PDIA6

Gene Symbol PDIA6
Gene Full Name protein disulfide isomerase family A, member 6
Background This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]
Function May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed:24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed:24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936). [UniProt]
Cellular Localization Endoplasmic reticulum lumen. Cell membrane. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). [UniProt]
Calculated MW 48 kDa