ARG40874
anti-PDK1 / Mitochondrial Pyruvate Dehydrogenase Kinase 1 antibody
anti-PDK1 / Mitochondrial Pyruvate Dehydrogenase Kinase 1 antibody for Western blot,Immunoprecipitation and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDK1 / Mitochondrial Pyruvate Dehydrogenase Kinase 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDK1 / Mitochondrial Pyruvate Dehydrogenase Kinase 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human PDK1. |
| Conjugation | Un-conjugated |
| Alternate Names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q15118 Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochond Swiss-port # Q8BFP9 Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochond |
|---|---|
| Gene Symbol | PDK1 |
| Gene Full Name | pyruvate dehydrogenase kinase, isozyme 1 |
| Background | Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013] |
| Function | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. [UniProt] |
| Cellular Localization | Mitochondrion matrix. [UniProt] |
| Calculated MW | 49 kDa |
| PTM | Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect. [UniProt] |
