ARG40833

anti-PDK2 antibody

anti-PDK2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Immunocytochemistry and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDK2
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Bov
Tested Application ICC, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDK2
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 379-392 of Human PDK2. (WRHYQTIQEAGDWC)
Conjugation Un-conjugated
Alternate Names PDHK2; PDKII; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII

Application Instructions

Application Suggestion
Tested Application Dilution
ICC1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5164 Human PDK2

GeneID: 81530 Rat PDK2

Swiss-port # Q15119 Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochond

Swiss-port # Q64536 Rat [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondri

Gene Symbol PDK2
Gene Full Name pyruvate dehydrogenase kinase, isozyme 2
Background This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Function Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. [UniProt]
Cellular Localization Mitochondrion matrix. [UniProt]
Calculated MW 46 kDa