ARG11125

anti-PEA15 antibody [4D2]

anti-PEA15 antibody [4D2] for Western blot,IHC-Frozen sections and Rat,Mouse,Cow

Overview

Product Description Mouse Monoclonal antibody [4D2] recognizes PEA15
Tested Reactivity Ms, Rat, Cow
Predict Reactivity Hu
Tested Application IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 4D2
Isotype IgG1
Target Name PEA15
Antigen Species Human
Immunogen Full-length Human PEA15.
Conjugation Un-conjugated
Alternate Names MAT1H; MAT1; Astrocytic phosphoprotein PEA-15; HUMMAT1H; 15 kDa phosphoprotein enriched in astrocytes; PED; PEA-15; Phosphoprotein enriched in diabetes; HMAT1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:1000 - 1:2000
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 15 kDa

Properties

Form Liquid
Purification Purified
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 364052 Rat PEA15

Swiss-port # Q5U318 Rat Astrocytic phosphoprotein PEA-15

Gene Symbol PEA15
Gene Full Name phosphoprotein enriched in astrocytes 15
Background This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Function Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface. [UniProt]
Cellular Localization Cytoplasm. Note=Associated with microtubules. [UniProt]
Calculated MW 15 kDa
PTM Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones. [UniProt]