ARG43919

anti-PGK2 antibody

anti-PGK2 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PGK2
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PGK2
Antigen Species Human
Immunogen Human PGK2 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Phosphoglycerate kinase 2
Alternate Names PGK2; Phosphoglycerate Kinase; Phosphoglycerate Kinase, Testis Specific; PGKPS; EC 2.7.2.3; PGK-2; PGKB; Phosphoglycerate Kinase Autosomal Pseudogene; Epididymis Secretory Protein Li 272; Testicular Tissue Protein Li 139; DJ417L20.2; HEL-S-272

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18663 Mouse PGK2

GeneID: 5232 Human PGK2

Swiss-port # P07205 Human Phosphoglycerate kinase 2

Swiss-port # P09041 Mouse Phosphoglycerate kinase 2

Gene Symbol PGK2
Gene Full Name Phosphoglycerate Kinase 2
Background This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
Function Essential for sperm motility and male fertility.
Cellular Localization Cytoplasm
Calculated MW 45 kDa
PTM Acetylation, Phosphoprotein