ARG54446
anti-PHAP3 antibody
anti-PHAP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Immune System antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PHAP3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Specificity | This antibody recognizes human, mouse and rat PHAP III (35kDa). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PHAP3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to amino acids near the carboxy terminus of human PHAP III (accession no. NP_112182) |
| Conjugation | Un-conjugated |
| Alternate Names | LANPL; LANP-like protein; Acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-L |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549, HepG2 and Human small intestine |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANP32E |
| Gene Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member E |
| Background | The PHAP proteins (tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation. |
| Function | Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 31 kDa |
| PTM | Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity). |
