ARG66932
anti-PHOX2B antibody [SQab22261]
anti-PHOX2B antibody [SQab22261] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab22261] recognizes PHOX2B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab22261 |
| Isotype | IgG |
| Target Name | PHOX2B |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human PHOX2B. |
| Conjugation | Un-conjugated |
| Protein Full Name | Paired mesoderm homeobox protein 2B |
| Alternate Names | NBPhox; PHOX2B homeodomain protein; Paired-like homeobox 2B; Neuroblastoma Phox; Paired mesoderm homeobox protein 2B; PMX2B; NBLST2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 40 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human neuroblastoma tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99453 Human Paired mesoderm homeobox protein 2B |
|---|---|
| Gene Symbol | PHOX2B |
| Gene Full Name | paired-like homeobox 2b |
| Background | The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq, Jul 2008] |
| Function | Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 32 kDa |
