ARG63599
anti-PIK3C2A antibody
anti-PIK3C2A antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PIK3C2A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Dog |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PIK3C2A |
| Antigen Species | Human |
| Immunogen | C-TVKWYQLTAATYL |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.1.154; PI3K-C2-alpha; CPK; PI3-K-C2(ALPHA); Phosphoinositide 3-kinase-C2-alpha; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; PtdIns-3-kinase C2 subunit alpha; PI3-K-C2A |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00443 Human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alp |
|---|---|
| Background | The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 191 kDa |
| PTM | Phosphorylated upon insulin stimulation; which may lead to enzyme activation (By similarity). Phosphorylated on Ser-259 during mitosis and upon UV irradiation; which does not change enzymatic activity but leads to proteasomal degradation. Ser-259 phosphorylation may be mediated by CDK1 or JNK, depending on the physiological state of the cell. |
