ARG45078
anti-PKC theta antibody [M217]
anti-PKC theta antibody [M217] for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [M217] recognizes PKC theta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M217 |
| Isotype | IgG2a |
| Target Name | PKC theta |
| Antigen Species | Mouse |
| Immunogen | Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members. |
| Conjugation | Un-conjugated |
| Alternate Names | PRKCQ; Protein Kinase C Theta ; Protein Kinase C Theta Type; EC 2.7.11.13; NPKC-Theta; PRKCT; Protein Kinase C, Theta; EC 2.7.11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A Purified. |
| Buffer | PBS, 0.05% NaN3, 50% Glycerol and 0.1 % BSA. |
| Stabilizer | 50% Glycerol and 0.1 % BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRKCQ |
| Gene Full Name | Protein Kinase C Theta |
| Background | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008] |
| Function | Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking. [Uniprot] |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane. [Uniprot] |
| Calculated MW | 82 kDa |
| PTM | Phosphoprotein. [Uniprot] |
