ARG56998
anti-PKLR antibody [1E3]
anti-PKLR antibody [1E3] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1E3] recognizes PKLR |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1E3 |
| Isotype | IgG1, kappa |
| Target Name | PKLR |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 47-574 of Human PKLR. |
| Conjugation | Un-conjugated |
| Alternate Names | PKR; PKRL; PK1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Pyruvate kinase PKLR; Pyruvate kinase 1; EC 2.7.1.40; RPK; Red cell/liver pyruvate kinase; PKL |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PKLR |
| Gene Full Name | pyruvate kinase, liver and RBC |
| Background | The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Plays a key role in glycolysis. [UniProt] |
| Calculated MW | 62 kDa |
